Transformation and laccase mutant isolation inCoprinus congregatusby restriction enzyme-mediated integration
نویسندگان
چکیده
منابع مشابه
Transformation of the edible basidiomycete Lentinus edodes by restriction enzyme-mediated integration of plasmid DNA.
We have used the restriction enzyme-mediated DNA integration (REMI) method to establish a transformation system in Lentinus edodes using the recombinant plasmid pLC1-hph, which contains the L. edodes transcriptional signals and an Escherichia coli hygromycin B phosphotransferase gene. Protoplasts of L. edodes were treated by the PEG transformation mixture containing 50 units of SalI, which clea...
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Introduction of restriction enzyme along with linearized plasmid results in integration of plasmid DNA at genomic restriction sites in a high proportion of the resulting transformants. We have found that electroporating BamHI or EcoRI together with pyr5-6 plasmids cut with the same enzyme stimulates the efficiency of transformation in Dictyostelium discoideum more than 20-fold over the rate see...
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Laccase is an enzyme that has potential ability of oxidation. It belongs to those enzymes, which have innate properties of reactive radical production, and its utilization in many fields has been ignored because of its unavailability in the commercial field. There are diverse sources of laccase producing organisms like bacteria, fungi and plants. Textile, pulp and paper industries discharge a h...
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This study reports the isolation of novel Bacterium from Soil for production of Extra-cellular Laccase Enzyme. Samples from garden, ant-induced and animal dung contaminated soil were collected in the Devi Ahilya University campus and around, Indore, Madhya Pradesh. Various methods including biochemical and morphological identification for high productivity of the enzyme were used. The media opt...
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BACKGROUND Laccases have good potential as bioremediating agents and can be used continuously in the immobilized form like many other enzymes. METHODS In the present study, laccase from Cyathus bulleri was immobilized by entrapment in Poly Vinyl Alcohol (PVA) beads cross-linked with either nitrate or boric acid. Immobilized laccase was used for dye decolorization in both batch and continuous ...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1999
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.1999.tb13446.x